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pgl3 basic luciferase reporter backbone  (Addgene inc)


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    Structured Review

    Addgene inc pgl3 basic luciferase reporter backbone
    Pgl3 Basic Luciferase Reporter Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl3+basic+luciferase+reporter+backbone/pJC6-GL3+(Plasmid+%2311979)/pmc08292318-298-39-45
    Average 93 stars, based on 7 article reviews
    pgl3 basic luciferase reporter backbone - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: TGFβ promotes fibrosis by MYST1-dependent epigenetic regulation of autophagy
    Article Snippet: .. The following expression and reporter plasmids were used: adenoviral expression constructs encodings murine Beclin1 (Vector Biolabs, Malvern, PA, USA), murine Myst1 (Applied Biological Materials, Richmond, Canada), In addition, we cloned the MYST1 promoter (−684 bp to +7 bp) into pGL3-basic luciferase reporter backbone (Catalog #11979; Addgene, Watertown, MA, USA) (pGL3- MYST1 -promoter) and mutated three predicted SBEs in the promoter sequence at residues -279 to -282 (SBE1), at residues -384 to -387 (SBE2) and at residues -574 to -577 (SBE3) either individually or in combination by exchanging GTCT to ATGT (pGL3- MYST1 -ATGT) using the QuickChange Multi site-directed mutagenesis kit (Catalog# 200514; Agilent Technologies, Santa Clara, CA, USA). ..

    Construct:

    Article Title: TGFβ promotes fibrosis by MYST1-dependent epigenetic regulation of autophagy
    Article Snippet: .. The following expression and reporter plasmids were used: adenoviral expression constructs encodings murine Beclin1 (Vector Biolabs, Malvern, PA, USA), murine Myst1 (Applied Biological Materials, Richmond, Canada), In addition, we cloned the MYST1 promoter (−684 bp to +7 bp) into pGL3-basic luciferase reporter backbone (Catalog #11979; Addgene, Watertown, MA, USA) (pGL3- MYST1 -promoter) and mutated three predicted SBEs in the promoter sequence at residues -279 to -282 (SBE1), at residues -384 to -387 (SBE2) and at residues -574 to -577 (SBE3) either individually or in combination by exchanging GTCT to ATGT (pGL3- MYST1 -ATGT) using the QuickChange Multi site-directed mutagenesis kit (Catalog# 200514; Agilent Technologies, Santa Clara, CA, USA). ..

    Clone Assay:

    Article Title: TGFβ promotes fibrosis by MYST1-dependent epigenetic regulation of autophagy
    Article Snippet: .. The following expression and reporter plasmids were used: adenoviral expression constructs encodings murine Beclin1 (Vector Biolabs, Malvern, PA, USA), murine Myst1 (Applied Biological Materials, Richmond, Canada), In addition, we cloned the MYST1 promoter (−684 bp to +7 bp) into pGL3-basic luciferase reporter backbone (Catalog #11979; Addgene, Watertown, MA, USA) (pGL3- MYST1 -promoter) and mutated three predicted SBEs in the promoter sequence at residues -279 to -282 (SBE1), at residues -384 to -387 (SBE2) and at residues -574 to -577 (SBE3) either individually or in combination by exchanging GTCT to ATGT (pGL3- MYST1 -ATGT) using the QuickChange Multi site-directed mutagenesis kit (Catalog# 200514; Agilent Technologies, Santa Clara, CA, USA). ..

    Luciferase:

    Article Title: TGFβ promotes fibrosis by MYST1-dependent epigenetic regulation of autophagy
    Article Snippet: .. The following expression and reporter plasmids were used: adenoviral expression constructs encodings murine Beclin1 (Vector Biolabs, Malvern, PA, USA), murine Myst1 (Applied Biological Materials, Richmond, Canada), In addition, we cloned the MYST1 promoter (−684 bp to +7 bp) into pGL3-basic luciferase reporter backbone (Catalog #11979; Addgene, Watertown, MA, USA) (pGL3- MYST1 -promoter) and mutated three predicted SBEs in the promoter sequence at residues -279 to -282 (SBE1), at residues -384 to -387 (SBE2) and at residues -574 to -577 (SBE3) either individually or in combination by exchanging GTCT to ATGT (pGL3- MYST1 -ATGT) using the QuickChange Multi site-directed mutagenesis kit (Catalog# 200514; Agilent Technologies, Santa Clara, CA, USA). ..

    Sequencing:

    Article Title: TGFβ promotes fibrosis by MYST1-dependent epigenetic regulation of autophagy
    Article Snippet: .. The following expression and reporter plasmids were used: adenoviral expression constructs encodings murine Beclin1 (Vector Biolabs, Malvern, PA, USA), murine Myst1 (Applied Biological Materials, Richmond, Canada), In addition, we cloned the MYST1 promoter (−684 bp to +7 bp) into pGL3-basic luciferase reporter backbone (Catalog #11979; Addgene, Watertown, MA, USA) (pGL3- MYST1 -promoter) and mutated three predicted SBEs in the promoter sequence at residues -279 to -282 (SBE1), at residues -384 to -387 (SBE2) and at residues -574 to -577 (SBE3) either individually or in combination by exchanging GTCT to ATGT (pGL3- MYST1 -ATGT) using the QuickChange Multi site-directed mutagenesis kit (Catalog# 200514; Agilent Technologies, Santa Clara, CA, USA). ..

    Mutagenesis:

    Article Title: TGFβ promotes fibrosis by MYST1-dependent epigenetic regulation of autophagy
    Article Snippet: .. The following expression and reporter plasmids were used: adenoviral expression constructs encodings murine Beclin1 (Vector Biolabs, Malvern, PA, USA), murine Myst1 (Applied Biological Materials, Richmond, Canada), In addition, we cloned the MYST1 promoter (−684 bp to +7 bp) into pGL3-basic luciferase reporter backbone (Catalog #11979; Addgene, Watertown, MA, USA) (pGL3- MYST1 -promoter) and mutated three predicted SBEs in the promoter sequence at residues -279 to -282 (SBE1), at residues -384 to -387 (SBE2) and at residues -574 to -577 (SBE3) either individually or in combination by exchanging GTCT to ATGT (pGL3- MYST1 -ATGT) using the QuickChange Multi site-directed mutagenesis kit (Catalog# 200514; Agilent Technologies, Santa Clara, CA, USA). ..



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